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fluorescein conjugated streptavidin cy5  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fluorescein conjugated streptavidin cy5
    Fluorescein Conjugated Streptavidin Cy5, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescein+avidin/Fluorescein+Avidin+DCS%2C+Cell+Sorting+Grade/pm41922652-277-5-8
    Average 95 stars, based on 414 article reviews
    fluorescein conjugated streptavidin cy5 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Intracellular targets of RGDS peptide in melanoma cells
    Article Snippet: .. Cells were fixed with 3% paraformaldehyde in PBS, pH 7.4, for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS, pH 7.4, for 5 minutes at RT, and blocked for 30 minutes with BSA 2% in PBS, pH 7.4, at RT, followed by incubation with fluorescein avidin (1:40; Vector-Laboratories, Peterborough, UK) in PBS, pH 7.4, for 1 h at RT. .. After washing in 0.3% Triton X-100 in PBS, cells were incubated with PI at a final concentration of 5 μg/ml to visualize nuclei and analyzed using a Zeiss LSM 510 meta-confocal microscope (Zeiss).

    Article Title: An Antagomir to MicroRNA Let7f Promotes Neuroprotection in an Ischemic Stroke Model
    Article Snippet: .. Sections were washed in 0.2× SSC at RT for 30 min and then subject to three 5 min washes with PBS followed by incubation with Rhodamine Avidin or Fluorescein Avidin (1∶500, Vector, Burlingame, CA), for 1 h at RT. .. Following three PBS washes the sections were incubated in the blocking solution (NGS, triton, RNasin and 4 drops/ml of block of avidin blocking solution) at RT for 1 h and then incubated ON with either anti-GFAP (Sigma, St. Louis, MO), anti-CD11b (1∶100, Serotec, Raleigh, NC) or anti-Iba1 (1∶100; Abcam, San Francisco, CA).

    Article Title: Glutaminase Immunoreactivity and Enzyme Activity Is Increased in the Rat Dorsal Root Ganglion Following Peripheral Inflammation
    Article Snippet: The tissue was washed three times in PBS and incubated in biotinylated goat anti-rabbit IgG secondary antibody (3 μ g/mL; Vector) in PBS-T for 1 hr at room temperature. .. Sections were washed two times in PBS following secondary antibody incubation, washed in sodium carbonate buffered saline (SCBS), pH 8.5, incubated in fluorescein-avidin (1.5 μ g/mL; Vector) in SCBS for 1 hr, and washed three times in PBS. .. Coverslips were apposed with Vectashield mounting media (Vector) to retard fading of immunofluorescence.

    Article Title: Inhibitory Neuron and Hippocampal Circuit Dysfunction in an Aged Mouse Model of Alzheimer's Disease
    Article Snippet: Sections were incubated with biotinylated goat anti-rabbit antibody (Vector Laboratories, Burlingame, CA) for 60 min, washed with TBST, and then incubated with Texas Red Avidin DCS (Vector Laboratories, Burlingame, CA) for 20 minutes. .. Sections were washed with TBST, re-blocked using the avidin/biotin blocking kit, subjected to second round of serum blocking, and then incubated with a second round of antibodies, followed by washing and incubation with Fluorescein Avidin (Vector Laboratories, Burlingame, CA) for 10 min. .. Sections were mounted in Vectashield media (Vector Laboratories), and viewed under a confocal microscope (Olympus IX61 DSU).

    Article Title: Loss of tight junction protein claudin 18 uncovers alveolar epithelial stem cell plasticity and emergence of non-fibrogenic transitional progenitors
    Article Snippet: Following antigen retrieval in Antigen Unmasking Solution at high pH (Vector Laboratories, Newark, California, USA), lung sections were incubated in CAS block (Invitrogen, Carlsbad, California, USA), and then with primary antibodies overnight at 4° C. Anti-pro-SPC (Seven Hills BioReagents, Cincinnati, Ohio, USA, #9337, 1:500), -AQP5 (Alomone labs, Jerusalem, Israel, #AQP-005, 1:250), -KRT8 Troma 1 (DSHB, Iowa City, Iowa, USA, Supernatant 1mL, 1:30), - KRT19 Troma 3 (DSHB Supernatant 1mL, 1:50), and -tdTomato (SICGEN, Cantanhede, Portugal, #AB8181, 1:50) were used as primary antibodies. .. Slides were then incubated with rat biotin (Vector Laboratories #BA-9400), Fluorescein avidin (Vector Laboratories, #A-2011), and rabbit 647 (Invitrogen, #A32733), goat 594 (Abcam, Cambridge, United Kingdom, #ab150132), or mouse 647 (Invitrogen, #A32787) Alexa Fluor secondary antibodies. .. Normal IgGs (Vector), were used as negative controls.

    Avidin-Biotin Assay:

    Article Title: Intracellular targets of RGDS peptide in melanoma cells
    Article Snippet: .. Cells were fixed with 3% paraformaldehyde in PBS, pH 7.4, for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS, pH 7.4, for 5 minutes at RT, and blocked for 30 minutes with BSA 2% in PBS, pH 7.4, at RT, followed by incubation with fluorescein avidin (1:40; Vector-Laboratories, Peterborough, UK) in PBS, pH 7.4, for 1 h at RT. .. After washing in 0.3% Triton X-100 in PBS, cells were incubated with PI at a final concentration of 5 μg/ml to visualize nuclei and analyzed using a Zeiss LSM 510 meta-confocal microscope (Zeiss).

    Article Title: The Vitamin K-Dependent Anticoagulant Factor, Protein S, Regulates Vascular Permeability.
    Article Snippet: .. Following fixation, cells were permeabilized in 0.1% Triton in PBS for 5 min and blocked with 0.5% BSA (Bovine Serum Albumin) in PBS for 30 min. A mix of biotinylated anti-mouse and rabbit antibodies and Fluorescein Avidin (all from Vector Laboratories; Peterborough, UK) Curr. ..

    Article Title: A new hexaploid wheat species Triticum aminovii
    Article Snippet: The hexaploid wheat (Triticum aestivum L., genome BBAADD, 2n = 6x = 42) is one of the most important food crops.. Enhancement and preservation its biodiversity is a key issue in the agrobiology of the twenty-first century.. Related cultivated and wild species are the most practical source of this enhancement.

    Article Title: An Antagomir to MicroRNA Let7f Promotes Neuroprotection in an Ischemic Stroke Model
    Article Snippet: .. Sections were washed in 0.2× SSC at RT for 30 min and then subject to three 5 min washes with PBS followed by incubation with Rhodamine Avidin or Fluorescein Avidin (1∶500, Vector, Burlingame, CA), for 1 h at RT. .. Following three PBS washes the sections were incubated in the blocking solution (NGS, triton, RNasin and 4 drops/ml of block of avidin blocking solution) at RT for 1 h and then incubated ON with either anti-GFAP (Sigma, St. Louis, MO), anti-CD11b (1∶100, Serotec, Raleigh, NC) or anti-Iba1 (1∶100; Abcam, San Francisco, CA).

    Article Title: Inhibitory Neuron and Hippocampal Circuit Dysfunction in an Aged Mouse Model of Alzheimer's Disease
    Article Snippet: Sections were incubated with biotinylated goat anti-rabbit antibody (Vector Laboratories, Burlingame, CA) for 60 min, washed with TBST, and then incubated with Texas Red Avidin DCS (Vector Laboratories, Burlingame, CA) for 20 minutes. .. Sections were washed with TBST, re-blocked using the avidin/biotin blocking kit, subjected to second round of serum blocking, and then incubated with a second round of antibodies, followed by washing and incubation with Fluorescein Avidin (Vector Laboratories, Burlingame, CA) for 10 min. .. Sections were mounted in Vectashield media (Vector Laboratories), and viewed under a confocal microscope (Olympus IX61 DSU).

    Article Title: The Vitamin K-Dependent Anticoagulant Factor, Protein S, Regulates Vascular Permeability
    Article Snippet: .. Following fixation, cells were permeabilized in 0.1% Triton in PBS for 5 min and blocked with 0.5% BSA (Bovine Serum Albumin) in PBS for 30 min. A mix of biotinylated anti-mouse and rabbit antibodies and Fluorescein Avidin (all from Vector Laboratories; Peterborough, UK) were used as secondary antibodies/reagents ( ). .. Confocal microscopy acquisitions were performed on a confocal FV-1000 station installed on an inverted microscope IX-81 (Olympus).

    Article Title: Loss of tight junction protein claudin 18 uncovers alveolar epithelial stem cell plasticity and emergence of non-fibrogenic transitional progenitors
    Article Snippet: Following antigen retrieval in Antigen Unmasking Solution at high pH (Vector Laboratories, Newark, California, USA), lung sections were incubated in CAS block (Invitrogen, Carlsbad, California, USA), and then with primary antibodies overnight at 4° C. Anti-pro-SPC (Seven Hills BioReagents, Cincinnati, Ohio, USA, #9337, 1:500), -AQP5 (Alomone labs, Jerusalem, Israel, #AQP-005, 1:250), -KRT8 Troma 1 (DSHB, Iowa City, Iowa, USA, Supernatant 1mL, 1:30), - KRT19 Troma 3 (DSHB Supernatant 1mL, 1:50), and -tdTomato (SICGEN, Cantanhede, Portugal, #AB8181, 1:50) were used as primary antibodies. .. Slides were then incubated with rat biotin (Vector Laboratories #BA-9400), Fluorescein avidin (Vector Laboratories, #A-2011), and rabbit 647 (Invitrogen, #A32733), goat 594 (Abcam, Cambridge, United Kingdom, #ab150132), or mouse 647 (Invitrogen, #A32787) Alexa Fluor secondary antibodies. .. Normal IgGs (Vector), were used as negative controls.

    Saline:

    Article Title: Glutaminase Immunoreactivity and Enzyme Activity Is Increased in the Rat Dorsal Root Ganglion Following Peripheral Inflammation
    Article Snippet: The tissue was washed three times in PBS and incubated in biotinylated goat anti-rabbit IgG secondary antibody (3 μ g/mL; Vector) in PBS-T for 1 hr at room temperature. .. Sections were washed two times in PBS following secondary antibody incubation, washed in sodium carbonate buffered saline (SCBS), pH 8.5, incubated in fluorescein-avidin (1.5 μ g/mL; Vector) in SCBS for 1 hr, and washed three times in PBS. .. Coverslips were apposed with Vectashield mounting media (Vector) to retard fading of immunofluorescence.

    Blocking Assay:

    Article Title: Inhibitory Neuron and Hippocampal Circuit Dysfunction in an Aged Mouse Model of Alzheimer's Disease
    Article Snippet: Sections were incubated with biotinylated goat anti-rabbit antibody (Vector Laboratories, Burlingame, CA) for 60 min, washed with TBST, and then incubated with Texas Red Avidin DCS (Vector Laboratories, Burlingame, CA) for 20 minutes. .. Sections were washed with TBST, re-blocked using the avidin/biotin blocking kit, subjected to second round of serum blocking, and then incubated with a second round of antibodies, followed by washing and incubation with Fluorescein Avidin (Vector Laboratories, Burlingame, CA) for 10 min. .. Sections were mounted in Vectashield media (Vector Laboratories), and viewed under a confocal microscope (Olympus IX61 DSU).



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